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KMID : 0364819910290010049
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Korean Journal of Microbiology
1991 Volume.29 No. 1 p.49 ~ p.55
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Synthesis and Thermotolerance of Heat Shock Proteins in Campylobacter jejuni
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김치경/Kim, Chi Kyung
김현옥/이길재/Kim, Hyun Ok/Lee, Kil Jae
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Abstract
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The heat shock responses of Campylobacter jejuni were studied by examination of their survival rates and synthesis of heat shock proteins. When C. jejuni cells were treated at the sublethal temperatures of 48C for 30 minutes, most of the cells maintained their viabilities and synthesized the heat shock proteins of 90, 73, and 66 kD in molecular weight. By the method of two-dimensional electrophoresis, the heat shock proteins of C jejuni were identified to be Hsp9O, Hsp73, and Hsp66. During the heat shock at 48C, the heat shock proteins were induced from about 5 minutes after the heat shock treatment. Their synthesis was continued upto 30 minutes, but remarkably retarded after 50 minutes. When C. jejuni cells were heat shocked at 51C for 30 minutes, the survival rates of the cells were decreased by about i0 fold and synthesis of heat shock proteins and normal proteins was also generally retarded. The cells exposed to 55C for 30 minutes died off by more than 10 cells and the new protein synthesis was not observed. But when C. jejuni cells were heat-shocked at the sublethal temperature of 48C for 15 to 20 minutes and then were exposed at the lethal temperature of 55C for 30 minutes, their viabilities were higher than those exposed at 55C for 30 minutes without pre-heat shock at 48C. Therefore, the heat shock proteins synthesized at the sublethal temperature of 48C in C. jejuni were thought to be responsible for thermotolerance. However, when C jejuni cells heat-shocked at various ranges of sublethal and lethal temperatures were placed back to the optimum temperature of 42C, the multiplication patterns of the cells pretreated at different temperatures were not much different each other.
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KEYWORD
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Heal shock response, Hsp synthesis, Thermotolerance, Campylbacter jejuni
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